Enterovirus Universal
Anaran'ny vokatra
HWTS-EV001- Enterovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiolojia
Ny aretin'ny tanana-foot-mouth dia areti-mifindra vokatry ny enterovirus (EV).Amin'izao fotoana izao, 108 karazana serotypes enteroviruses hita, izay mizara ho vondrona efatra: A, B, C ary D. Anisan'izany ny enterovirus EV71 sy CoxA16 no tena otrikaretina.Ny zaza latsaky ny 5 taona no tena voan’io aretina io, ary mety hiteraka herpes amin’ny tanana, tongotra, vava ary faritra hafa.Ankizy vitsivitsy dia mety hiteraka fahasarotana toy ny myocarditis, edema pulmonary, ary meningoencephalitis aseptika.
fantsona
| FAM | EV RNA |
| ROX | Fanaraha-maso anatiny |
Paramètre ara-teknika
| fitahirizana | ≤-18 ℃ |
| talantalana-fiainana | 12 volana |
| Karazana karazana | Oropharyngeal swab,Herpes fluid |
| Ct | ≤38 |
| CV | ≤5.0% |
| Loda | 500 kopia/mL |
| Fitaovana azo ampiharina | Applied Biosystems 7500/7500 Fast Real-Time PCR Systems, QuantStudio®5 Rafitra PCR amin'ny fotoana tena izy SLAN-96P Real-Time PCR Systems LightCycler®480 Rafitra PCR Real-Time LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Tena-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Rafitra PCR Real Time |
Fizotran'asa
Safidy 1.
Recommend Extraction Kit: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ary Macro & Micro-Test Automatic Nucleic Acid Extractor ( HWTS-3006B, HWTS-3006C), dia tokony hesorina tanteraka araka ny toromarika.Ny habetsaky ny santionany dia 200 μL, ny habetsaky ny elution atolotra dia 80μL.
Safidy 2.
Kitapo fitrandrahana atolotra: Macro & Micro-Test Sample Reagent Reagent (HWTS-3005-8), dia tokony hesorina araka ny toromarika.
Safidy3.
Kitapo fitrandrahana soso-kevitra: QIAamp Viral RNA Mini Kit (52904) na Nucleic Acid Extraction or Purification Kit (YDP315-R), dia tokony hesorina mifanaraka amin'ny toromarika.Ny habetsaky ny santionany dia 140 μL, ny habetsaky ny elution atolotra dia 60μL.
