Otrik'aretina gripa A Universal/H1/H3
Anaran'ny vokatra
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit (Fluorescence PCR)
Epidemiolojia
Ny viriosy gripa dia karazana solontenan'ny Orthomyxoviridae. Otrikaretina tena manimba ny fahasalaman'ny olombelona izy io. Mety hiparitaka be ny mpampiantrano azy. Manodidina ny 600 tapitrisa eran-tany ny valanaretina isan-taona ary miteraka fahafatesana 250.000 ~ 500.000, ka ny viriosy gripa A no tena mahatonga ny aretina sy ny fahafatesana. Ny otrik'aretina gripa A dia RNA miendrika tady miiba tokana. Araka ny hemagglutinin (HA) sy ny neuraminidase (NA), ny HA dia azo zaraina ho subtypes 16, NA Mizara ho subtype 9. Ao anatin'ireo viriosy gripa A, ny karazana viriosy gripa izay afaka miparitaka mivantana amin'ny olombelona dia: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 ary H10N8. Anisan'izany, ny subtypes H1 sy H3 dia tena pathogenic, ary tena mendrika ny hojerena.
fantsona
| FAM | gripa A universal karazana virosy nucleic asidra |
| VIC/HEX | gripa A H1 karazana virosy nucleic asidra |
| ROX | gripa A H3 karazana virosy nucleic asidra |
| CY5 | fanaraha-maso anatiny |
Paramètre ara-teknika
| fitahirizana | ≤-18 ℃ |
| talantalana-fiainana | 9 volana |
| Karazana karazana | nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| Loda | 500 kopia/μL |
| manokana | Tsy misy fifampikasohana amin'ny santionany amin'ny taovam-pisefoana hafa toy ny gripa A, gripa B, Legionella pneumophila, tazo Rickettsia Q, Chlamydia pneumoniae, Adenovirus, Virus Syncytial Respiratory, Parainfluenza 1, 2, 3 , Virus Coxsackie, Virus Echo, Metapneumovirus A1/B2, Virus Respiratory A1/B2 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, sns ary ny ADN génomika olombelona. |
| Fitaovana azo ampiharina | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Rafitra PCR Real Time SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Rafitra PCR Real-Time LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer teknolojia) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Rafitra PCR Real Time |
Fizotran'asa
Ny fitrandrahana asidra nucleic na ny fanadiovana fanadiovana (YDP315-R) nataon'i Tiangen Biotech (Beijing) Co., Ltd. Ny fitrandrahana dia tokony hatao araka ny torolàlana ampiasaina. Ny boky santionany nalaina dia 140μL, ary ny boky elution soso-kevitra dia 60μL.
Safidy 2.
Makro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ary Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ny fitrandrahana dia tokony hatao araka ny torolàlana ampiasaina. Ny boky santionany nalaina dia 200μL, ary ny boky elution soso-kevitra dia 80μL.
